Investigation of flow field characteristics and performance of carbon-hydrogen/oxygen-rich air rotating detonation engine

Numerical simulations were conducted to investigate the flow field characteristics and performance of a carbon-hydrogen/oxygen-rich air rotating detonation engine (RDE). Three distinct flow field structures were observed in the gas-solid two-phase RDE. The results show that reducing the hydrogen equivalence ratio and particle diameter both contribute to the transition from gas-phase single-front detonation to gas-solid two-phase double-front detonation and further to gas-solid two-phase single-front detonation. The effects of solid fuel particle diameter and hydrogen equivalence ratio on the flow field characteristics and performance are revealed. The results show that reducing the particle diameter enhances the speed of the two-phase detonation wave, improves the pressure gain in the combustion chamber, and increases the specific impulse. Decreasing the hydrogen equivalence ratio reduces the detonation wave speed, enhances the stability of the detonation flow field, increases the pressure gain in the detonation wave and combustion chamber and boosts thrust. Furthermore, the selection of operational conditions to ensure stable operation and optimal performance of the RDE is discussed. In order to take into account the requirements of stability, pressure gain performance and propulsion performance, two-phase single-front detonation should be realized in gas-solid two-phase RDE, and smaller hydrogen equivalent ratio and appropriate particle diameter should be selected. According to the conclusion of this study, the particle diameter should be 0.5-1 {\mu}m. Under such conditions, the detonation flow field demonstrates good stability, allowing the RDE to achieve higher pressure gain and specific impulse while maintaining stable operation

Bloodstream infection, peritonitis, and pneumonia caused by Pasteurella multocida in a patient with liver cirrhosis despite no animal contact: case report and literature review

Pasteurella multocida is an opportunistic pathogen. Previously reported infections associated with P. multocida have often been linked to contact with cats, dogs, and other animals. Cases of systemic multiple-site infections following P. multocida infection are rare. This case study presents a 49-year-old middle-aged man with post-hepatitis B cirrhosis and no history of animal contact. The patient was admitted with symptoms of fever accompanied by diarrhea, abdominal distension, and cough. Blood tests showed elevated levels of CRP, PCT, and IL-6, and blood culture revealed the growth of P. multocida. CT scans revealed a large amount of abdominal effusion, a small amount of pleural effusion, and pulmonary infection foci. The patient’s condition improved after successive administration of ceftriaxone and levofloxacin to fight the infection, and abdominal puncture and drainage. Multiple-site infections caused by P. multocida are rarely encountered in patients with liver cirrhosis but without animal contact, which could be regarded as serious conditions warranting careful attention in terms of clinical diagnosis and treatment

Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction

Abstract Background Acute myocardial infarction (AMI) is characterized by an inflammatory process in which T cell plays a key role. However, the profile of immune microenvironment in AMI is still uncertain. High-throughput sequencing of T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. Methods 30 patients with AMI were enrolled and 30 healthy individuals were recruited as controls. Flow cytometer were used to analyze the distribution of αβ T cells and their CD69 expression from peripheral leukomonocytes. TCRβ repertoire library was amplified by two-round multiplex PCR and detected by next-generation sequencing (NGS). Results The percentage of αβ T cells in AMI patients were significantly restricted than those in healthy controls, while the highly activated αβ T cells along with distinguishing usage of variable (V), diversity (D) and joining (J) gene segments were also found in AMI patients. In addition, AMI induced a significantly restricted CDR3 amino acid (AA) diversity and remarkably reconstituted TCR immune repertoires. Finally, we identified several AMI-associated tendency of CDR3 AAs expression after AMI. Conclusions Our work suggests that the aberrant αβ T cells distribution and activation may associated with the pathogenesis of AMI and demonstrates a reconstitution of TCRβ immune repertoire after AMI

First case of bloodstream infection caused by NDM-positive Escherichia hermannii

Abstract Background Escherichia hermannii (E. hermanni) is always accompanied by other bacterial infections in humans. In previous reports, most E. hermannii-related infections were caused by sensitive strains. Here, for the first time, we report the case of a patient with New Delhi metallo-β-lactamase (NDM)-positive E. hermannii bloodstream infection. Case presentation The patient was a 70-year-old male admitted to our hospital due to a 4-day fever, with a history of malignant tumor, liver cirrhosis, and chronic obstructive pulmonary disease. After admission, his blood culture tested positive for E. hermannii. The drug resistance analysis showed positive for NDM resistance, with susceptibility to aztreonam, levofloxacin, and amikacin. The blood culture turned negative after 8 days of aztreonam treatment. The patient’s symptoms improved, and he was discharged after 14 days of hospitalization. Conclusions This is the first report of a bloodstream infection caused by an NDM-positive E. hermannii strain. The anti-infection regimen used in this case provides a new reference regimen for clinical practice

A novel immune checkpoint score system for prognostic evaluation in pancreatic adenocarcinoma

Abstract Background Pancreatic adenocarcinoma (PAAD) remains a lethal malignancy making the detection of novel prognostic biomarkers urgent. Limited studies have investigated the predictive capability of immune checkpoints in PAAD. Method Gene expression data and correlative clinical information of PAAD cohort were obtained from public databases, including TCGA, ICGC, GTEX and GEO databases. Risk factors were screened and used to establish a risk score model through LASSO and Cox regression analyses. The prognostic ability of the risk score model was demonstrated. The association between risk score with immune cells infiltration, immune checkpoint genes expression, immunogenic cell death, somatic mutations and signaling pathways enrichment were analysed. scRNA-seq data were collected to confirmed the immune checkpoints expression in PAAD samples. The prognosis prediction ability of OX40/TNFRSF4 was identified. The mRNA and protein expression of OX40 in our clinical specimens were examined by RT-PCR and IHC method and its prognosis ability was verified. Results First of all, the difference of immune microenvironment between pancreatic cancer and adjacent tissues was shown. A risk score system based on three immune checkpoints (OX40, TNFSF14 and KIR3DL1) was established. The risk score model was an independent prognostic factor and performed well regarding overall survival (OS) predictions among PAAD patients. A nomogram was established to facilitate the risk model application in clinical prognosis. Immune cells including naive B cells, CD8+ T cells and Tregs were negatively correlated with the risk score. The risk score was associated with expression of immune checkpoint genes, immunogenic cell death related genes and somatic mutations. Glycolysis processes, IL-2-STAT5, IL-6-STAT3, and mTORC1 signaling pathways were enriched in the high-risk score group. Furthermore, scRNA-seq data confirmed that TNFRSF4, TNFSF14 and KIR3DL1 were expressed on immune cells in PAAD samples. We then identified OX40 as an independent prognosis-related gene, and a higher OX40 expression was associated with increased survival rate and immune environment change. In 84 PAAD clinical specimens collected from our center, we confirmed that higher OX40 mRNA expression levels were related to a good prognosis. The protein expression of OX40 on tumor-infiltrating immune cells (TIICs), endothelial cells and tumor cells was verified in PAAD tissues by immunohistochemistry (IHC) method. Conclusions Overall, our findings strongly suggested that the three-immune checkpoints score system might be useful in the prognosis and design of personalized treatments for PAAD patients. Finally, we identified OX40 as an independent potential biomarker for PAAD prognosis prediction

Development of a ZTD Vertical Profile Model Considering the Spatiotemporal Variation of Height Scale Factor with Different Reanalysis Products in China

Tropospheric delay is one of the key factors that may affect high-precision satellite navigation and positioning and water vapor retrieval performance. Its variation in the vertical direction is much greater than that in the horizontal direction. Therefore, the vertical profile model of zenith total delay (ZTD) is important for the spatial interpolation of high-precision ZTD products and the development of ZTD models. However, in China, low spatial and temporal resolutions remain persistent in ZTD vertical profile models and limit their application. In this study, ZTD vertical profile grid models (CZTD-H model: CZTD-HM and CZTD-HE models) were developed by considering the time-varying height scale factor for China and employing ZTD layered profile information with high temporal-spatial resolution calculated using MERRA-2 data and ERA5 data based on the integration method during 2012–2016. The CZTD-H model accuracy was verified using the global navigation satellite system (GNSS) data acquired from the Crustal Movement Observation Network of China (CMONOC) and radiosonde data as reference and was compared with the canonical GPT3 model accuracy. The applicability of CZTD-HM and CZTD-HE models was discussed. The results showed that: (1) CZTD-HM and CZTD-HE models exhibited excellent performance for ZTD layered vertical interpolation in northwestern and southeastern China, respectively. Among ZTD layered profiles from 84 radiosonde stations, the RMSE of ZTD data interpolated using CZTD-HM and CZTD-HE models improved by 12.9/16.23% and 13.8/17.16% compared with GPT3-1 and GPT3-5 models, respectively. (2) The CZTD-H model maintained high performance for the spatial interpolation of GGOS grid ZTD data. Validation with ZTD data from 249 GNSS stations showed that the RMSEs of both CZTD-HM and CZTD-HE models improved by 2.8 mm (19.7%) and 2.6 mm (18.6%) compared with those of the GPT3-1 and GPT3-5 models, respectively. The CZTD-HE model showed excellent performance in summer among all the models. Only the location and day of the year were required for the application of the CZTD-H model, which showed excellent ZTD vertical correction performance in China. With the different performances of the CZTD-HE and CZTD-HM models in China, the ERA5 model can be recommended for practical applications. Therefore, these results can provide a reference for the data source selection of ZTD vertical profile model construction on the basis of high-precision reanalysis data, GNSS real-time high-precision positioning, and GNSS meteorology in China